Articles in press have been peer-reviewed and accepted, which are not yet assigned to volumes /issues, but are citable by Digital Object Identifier (DOI).
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Sicien H. Chen, one of the pioneers and founders of Chinese entomology
Yejing Ge
2022, 13(5): 309-312.   doi: 10.1007/s13238-021-00829-8
[Abstract](193) [PDF 759KB](4)
Ferroptosis as an important driver of lupus
Chao Mao, Guang Lei, Li Zhuang, Boyi Gan
2022, 13(5): 313-315.   doi: 10.1007/s13238-021-00892-1
[Abstract](138) [PDF 272KB](12)
Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing
Xiya Zhang, Tao Li, Jianping Ou, Junjiu Huang, Puping Liang
2022, 13(5): 316-335.   doi: 10.1007/s13238-021-00838-7
[Abstract](211) [PDF 921KB](33)
Recent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics. CRISPR-Cas nucleases, together with the DNA damage repair pathway in cells, enable both genetic diversification by classical non-homologous end joining (c-NHEJ) and precise genome modification by homology-based repair (HBR). Genome editing in zygotes is a convenient way to edit the germline, paving the way for animal disease model generation, as well as human embryo genome editing therapy for some life-threatening and incurable diseases. HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template. Here, we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor. Moreover, we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos. Finally, we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.
Research Article
Cardioprotective mechanism of SGLT2 inhibitor against myocardial infarction is through reduction of autosis
Kai Jiang, Yue Xu, Dandan Wang, Feng Chen, Zizhuo Tu, Jie Qian, Sheng Xu, Yixiang Xu, John Hwa, Jian Li, Hongcai Shang, Yaozu Xiang
2022, 13(5): 336-359.   doi: 10.1007/s13238-020-00809-4
[Abstract](354) [PDF 9102KB](91)
Sodium-glucose cotransporter 2 (SGLT2) inhibitors reduce cardiovascular mortality in patients with diabetes mellitus but the protective mechanism remains elusive. Here we demonstrated that the SGLT2 inhibitor, Empagliflozin (EMPA), suppresses cardiomyocytes autosis (autophagic cell death) to confer cardioprotective effects. Using myocardial infarction (MI) mouse models with and without diabetes mellitus, EMPA treatment significantly reduced infarct size, and myocardial fibrosis, thereby leading to improved cardiac function and survival. In the context of ischemia and nutritional glucose deprivation where autosis is already highly stimulated, EMPA directly inhibits the activity of the Na+/H+ exchanger 1 (NHE1) in the cardiomyocytes to regulate excessive autophagy. Knockdown of NHE1 significantly rescued glucose deprivation-induced autosis. In contrast, overexpression of NHE1 aggravated the cardiomyocytes death in response to starvation, which was effectively rescued by EMPA treatment. Furthermore, in vitro and in vivo analysis of NHE1 and Beclin 1 knockout mice validated that EMPA’s cardioprotective effects are at least in part through downregulation of autophagic flux. These findings provide new insights for drug development, specifically targeting NHE1 and autosis for ventricular remodeling and heart failure after MI in both diabetic and non-diabetic patients.
The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication
Wan Zhao, Junjie Zhu, Hong Lu, Jiaming Zhu, Fei Jiang, Wei Wang, Lan Luo, Le Kang, Feng Cui
2022, 13(5): 360-378.   doi: 10.1007/s13238-021-00822-1
[Abstract](422) [PDF 13483KB](58)
Rice stripe virus (RSV) transmitted by the small brown planthopper causes severe rice yield losses in Asian countries. Although viral nuclear entry promotes viral replication in host cells, whether this phenomenon occurs in vector cells remains unknown. Therefore, in this study, we systematically evaluated the presence and roles of RSV in the nuclei of vector insect cells. We observed that the nucleocapsid protein (NP) and viral genomic RNAs were partially transported into vector cell nuclei by utilizing the importin α nuclear transport system. When blocking NP nuclear localization, cytoplasmic RSV accumulation significantly increased. In the vector cell nuclei, NP bound the transcription factor YY1 and affected its positive regulation to FAIM. Subsequently, decreased FAIM expression triggered an antiviral caspase-dependent apoptotic reaction. Our results reveal that viral nuclear entry induces completely different immune effects in vector and host cells, providing new insights into the balance between viral load and the immunity pressure in vector insects.
Comprehensive analysis of RNA-seq and whole genome sequencing data reveals no evidence for SARS-CoV-2 integrating into host genome
Yu-Sheng Chen, Shuaiyao Lu, Bing Zhang, Tingfu Du, Wen-Jie Li, Meng Lei, Yanan Zhou, Yong Zhang, Penghui Liu, Yong-Qiao Sun, Yong-Liang Zhao, Ying Yang, Xiaozhong Peng, Yun-Gui Yang
2022, 13(5): 379-385.   doi: 10.1007/s13238-021-00861-8
[Abstract](196) [PDF 945KB](35)
Current Issue
  • ISSN1674-800X
  • EISSN1674-8018
  • IF (2020) 14.87

Microbiome—Towards Translation

May 2021
Volume 12
Issue 5
pp: 315-435

DHODH inhibitors fight SARS-CoV-2

October 2020
Volume 11
Issue 10
pp: 699-782

Microbiota and Human Health

May 2018
Volume 9
Issue 5
pp: 395-510