Volume 2 Issue 1
Jan.  2011
Turn off MathJax
Article Contents
Bo-jiao Yin, Ting Gao, Nuo-yan Zheng, Yin Li, San-yuan Tang, Li-ming Liang, Qi XIE. Generation of glyco-engineered BY2 cell lines with decreased expression of plant-specific glycoepitopes[J]. Protein&Cell, 2011, 2(1): 41-47. doi: 10.1007/s13238-011-1007-4
Citation: Bo-jiao Yin, Ting Gao, Nuo-yan Zheng, Yin Li, San-yuan Tang, Li-ming Liang, Qi XIE. Generation of glyco-engineered BY2 cell lines with decreased expression of plant-specific glycoepitopes[J]. Protein&Cell, 2011, 2(1): 41-47. doi: 10.1007/s13238-011-1007-4

Generation of glyco-engineered BY2 cell lines with decreased expression of plant-specific glycoepitopes

doi: 10.1007/s13238-011-1007-4
Funds:

This work was supported by grants from National Natural Science Foundation of China (Grant No. 31030047) and the European Commission Sixth Framework Program (Grant No. SP22-CT-2004-511060).

  • Received Date: 2010-12-22
  • Rev Recd Date: 2011-01-06
  • Plants are known to be efficient hosts for the production of mammalian therapeutic proteins. However, plants produce complex N-glycans bearing β1,2-xylose and core α1,3-fucose residues, which are absent in mammals. The immunogenicity and allergenicity of plant-specific Nglycans is a key concern in mammalian therapy. In this study, we amplified the sequences of 2 plant-specific glycosyltransferases from Nicotiana tabacum L. cv Bright Yellow 2 (BY2), which is a well-established cell line widely used for the expression of therapeutic proteins. The expression of the endogenous xylosyltranferase (XylT) and fucosyltransferase (FucT) was downregulated by using RNA interference (RNAi) strategy. The xylosylated and core fucosylated N-glycans were significantly, but not completely, reduced in the glycoengineered lines. However, these RNAi-treated cell lines were stable and viable and did not exhibit any obvious phenotype. Therefore, this study may provide an effective and promising strategy to produce recombinant glycoproteins in BY2 cells with humanized N-glycoforms to avoid potential immunogenicity.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (82) PDF downloads(165) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return