Volume 2 Issue 1
Jan.  2011
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Lifen Wang, Yi Zhan, Eli Song, Yong Yu, Yaming Jiu, Wen Du, Jingze Lu, Pingsheng Liu, Pingyong Xu, Tao Xu. HID-1 is a peripheral membrane protein primarily associated with the medial- and trans-Golgi apparatus[J]. Protein&Cell, 2011, 2(1): 74-85. doi: 10.1007/s13238-011-1008-3
Citation: Lifen Wang, Yi Zhan, Eli Song, Yong Yu, Yaming Jiu, Wen Du, Jingze Lu, Pingsheng Liu, Pingyong Xu, Tao Xu. HID-1 is a peripheral membrane protein primarily associated with the medial- and trans-Golgi apparatus[J]. Protein&Cell, 2011, 2(1): 74-85. doi: 10.1007/s13238-011-1008-3

HID-1 is a peripheral membrane protein primarily associated with the medial- and trans-Golgi apparatus

doi: 10.1007/s13238-011-1008-3
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This work was supported by grants from the National Science Foundation of China (Grant Nos. 30870564, and 30900268), The Beijing Natural Science Foundation (No. 5092017), the Major State Basic Research Program of China (No. 2010CB833701), and the CAS Project (KSCX2-SW-224 and Novo Nordisk-CAS to P Xu).

  • Received Date: 2011-01-11
  • Rev Recd Date: 2011-01-18
  • Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact that the hid-1 gene encodes a novel protein (HID-1) which is highly conserved from Caenorhabditis elegans to mammals, the domain structure, subcellular localization, and exact function of HID-1 remain unknown. Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain. In this study, we revealed that mammalian HID-1 localized to the medial- and trans-Golgi apparatus as well as the cytosol, and the localization was sensitive to brefeldin A treatment. Next, we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol. Finally, we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus. We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.
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