Volume 3 Issue 12
Dec.  2012
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Wei Li, Xinlu Wang, Guangxia Gao. Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA[J]. Protein&Cell, 2012, 3(12): 943-949. doi: 10.1007/s13238-012-2090-x
Citation: Wei Li, Xinlu Wang, Guangxia Gao. Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA[J]. Protein&Cell, 2012, 3(12): 943-949. doi: 10.1007/s13238-012-2090-x

Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA

doi: 10.1007/s13238-012-2090-x
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This work was supported in part by Grants (to G.G.) from National Natural Science Foundation of China (Grant Nos. 81030030, 81101262 and 81028011) and the National Basic Research Program (973 Program) (No. 2012CB910203).

  • Received Date: 2012-08-27
  • Rev Recd Date: 2012-09-04
  • Murine leukemia virus (MLV)-based retroviral vectors is widely used for gene transfer and basic research, and production of high-titer retroviral vectors is very important. Here we report that expression of the Y-box binding protein 1 (YB-1) enhanced the production of infectious MLV vectors. YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells, and thus increasing viral RNA levels in both producer cells and virion particles. The viral element responsive to YB-1 was mapped to the repeat sequence (R region) in MLV genomic RNA. These results identified YB-1 as a MLV mRNA stabilizer, which can be used for improving production of MLV vectors.
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