Volume 4 Issue 3
Mar.  2013
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Yu-Chung Chang, Hao Zhang, Mark L. Brennan, Jinhua Wu. Crystal structure of Lamellipodin implicates diverse functions in actin polymerization and Ras signaling[J]. Protein&Cell, 2013, 4(3): 211-219. doi: 10.1007/s13238-013-2082-5
Citation: Yu-Chung Chang, Hao Zhang, Mark L. Brennan, Jinhua Wu. Crystal structure of Lamellipodin implicates diverse functions in actin polymerization and Ras signaling[J]. Protein&Cell, 2013, 4(3): 211-219. doi: 10.1007/s13238-013-2082-5

Crystal structure of Lamellipodin implicates diverse functions in actin polymerization and Ras signaling

doi: 10.1007/s13238-013-2082-5
  • Received Date: 2012-08-25
  • Rev Recd Date: 2012-09-20
  • The adapter protein Lamellipodin (Lpd) plays an important role in cell migration. In particular, Lpd mediates lamellipodia formation by regulating actin dynamics via interacting with Ena/VASP proteins. Its RA-PH tandem domain configuration suggests that like its paralog RIAM, Lpd may also mediate particular Ras GTPase signaling. We determined the crystal structures of the Lpd RA-PH domains alone and with an N-terminal coiled-coil region (cc-RA-PH). These structures reveal that apart from the anticipated coiled-coil interaction, Lpd may also oligomerize through a second intermolecular contact site. We then validated both oligomerization interfaces in solution by mutagenesis. A fluorescence-polarization study demonstrated that Lpd binds phosphoinositol with low affinity. Based on our crystallographic and biochemical data, we propose that Lpd and RIAM serve diverse functions:Lpd plays a predominant role in regulating actin polymerization, and its function in mediating Ras GTPase signaling is largely suppressed compared to RIAM.
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