Homologous recombination in human embryonic stem cells using CRISPR/Cas9 nickase and a long DNA donor template

doi: 10.1007/s13238-014-0032-5

• Zhili Rong² , 
• Shengyun Zhu¹ , 
• Yang Xu² , 
• Xuemei Fu^{1,3 ,} 

• 1 Shenzhen Children's Hospital, 7019 Yitian Road, Shenzhen 518026, China;

• 2 Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA;

• 3 Chongqing Medical University, Chongqing 400016, China