Volume 8 Issue 8
Aug.  2017
Turn off MathJax
Article Contents
Xuyuan Zhang, Pan Yang, Nan Wang, Jialong Zhang, Jingyun Li, Hao Guo, Xiangyun Yin, Zihe Rao, Xiangxi Wang, Liguo Zhang. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection[J]. Protein&Cell, 2017, 8(8): 590-600. doi: 10.1007/s13238-017-0405-7
Citation: Xuyuan Zhang, Pan Yang, Nan Wang, Jialong Zhang, Jingyun Li, Hao Guo, Xiangyun Yin, Zihe Rao, Xiangxi Wang, Liguo Zhang. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection[J]. Protein&Cell, 2017, 8(8): 590-600. doi: 10.1007/s13238-017-0405-7

The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection

doi: 10.1007/s13238-017-0405-7
Funds:

This work was supported in part by grants from the National Natural Science Foundation of China (Grant Nos. 31670924 and 31570717), the National Basic Research Program (973 Program) (No. 2014CB542800) and the Open Research Project (2015kf04) from the State Key Laboratory of Biological Macromolecules of the Institute of Biophysics at the Chinese Academy of Sciences. X. Wang was supported by the Young Elite Scientist Sponsorship by CAST.

  • Received Date: 2017-02-22
  • Rev Recd Date: 2017-03-16
  • Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (190) PDF downloads(1512) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return