Volume 10 Issue 4
Apr.  2019
Turn off MathJax
Article Contents
Yirui Cheng, Qingyu Zeng, Qing Han, Weiliang Xia. Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes[J]. Protein&Cell, 2019, 10(4): 295-299. doi: 10.1007/s13238-018-0529-4
Citation: Yirui Cheng, Qingyu Zeng, Qing Han, Weiliang Xia. Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes[J]. Protein&Cell, 2019, 10(4): 295-299. doi: 10.1007/s13238-018-0529-4

Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes

doi: 10.1007/s13238-018-0529-4
Funds:

This study is supported by the National Natural Science Foundation of China (Grant No. 81773115), SJTU interdisciplinary grant (YG2017MS52), and by grants from Ministry of Science & Technology (2016YFC0906400).

  • loading
  • [1]
    Akers JC, Ramakrishnan V, Yang I, Hua W, Mao Y, Carter BS, Chen CC (2016) Optimizing preservation of extracellular vesicular miRNAs derived from clinical cerebrospinal fluid. Cancer Biomark 17:125-132
    [2]
    Ban JJ, Lee M, Im W, Kim M (2015) Low pH increases the yield of exosome isolation. Biochem Biophys Res Commun 461:76-79
    [3]
    Colombo M, Raposo G, Thery C (2014) Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol 30:255-289
    [4]
    Lee M, Ban JJ, Im W, Kim M (2016) Influence of storage condition on exosome recovery. Biotechnol Bioproc E 21:299-304
    [5]
    Mincheva-Nilsson L, Baranov V, Nagaeva O, Dehlin E (2016) Isolation and characterization of exosomes from cultures of tissue explants and cell lines. Curr Protoc Immunol 115:14-42
    [6]
    Parolini I, Federici C, Raggi C, Lugini L, Palleschi S, De Milito A, Coscia C, Iessi E, Logozzi M, Molinari A et al (2009) Microenvironmental pH is a key factor for exosome traffic in tumor cells. J Biol Chem 284:34211-34222
    [7]
    Qi HZ, Liu CY, Long LX, Ren Y, Zhang SS, Chang XD, Qian XM, Jia HH, Zhao J, Sun JJ et al (2016) Blood exosomes endowed with magnetic and targeting properties for cancer therapy. ACS Nano 10:3323-3333
    [8]
    Rider MA, Hurwitz SN, Meckes DG Jr (2016) ExtraPEG:a polyethylene glycol-based method for enrichment of extracellular vesicles. Sci Rep 6:23978
    [9]
    Shao Y, Shen Y, Chen T, Xu F, Chen X, Zheng S (2016) The functions and clinical applications of tumor-derived exosomes. Oncotarget 7:60736-60751
    [10]
    Sokolova V, Ludwig AK, Hornung S, Rotan O, Horn PA, Epple M, Giebel B (2011) Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy. Colloids Surf B Biointerfaces 87:146-150
    [11]
    Thery C, Amigorena S, Raposo G, Clayton A (2006) Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol Chapter 3, Unit 3-22
    [12]
    Tkach M, Thery C (2016) Communication by extracellular vesicles:where we are and where we need to go. Cell 164:1226-1232
    [13]
    Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO (2007) Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 9:654-U672
    [14]
    Zhao Y, Chen K, Li H, Wu H (2017) Effect of pH on the isolation of urinary exosome. Int Urol Nephrol 49:165-169
    [15]
    Zhou H, Yuen PS, Pisitkun T, Gonzales PA, Yasuda H, Dear JW, Gross P, Knepper MA, Star RA (2006) Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int 69:1471-1476
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)

    Article Metrics

    Article views (674) PDF downloads(1636) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return