Volume 3 Issue 1
Jan.  2012
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Qihui Wang, Xiaoning Mou, Henghua Cao, Qingzhang Meng, Yanni Ma, Pengcheng Han, Junjie Jiang, Hao Zhang, Yue Ma. A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture[J]. Protein&Cell, 2012, 3(1): 51-59. doi: 10.1007/s13238-012-2002-0
Citation: Qihui Wang, Xiaoning Mou, Henghua Cao, Qingzhang Meng, Yanni Ma, Pengcheng Han, Junjie Jiang, Hao Zhang, Yue Ma. A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture[J]. Protein&Cell, 2012, 3(1): 51-59. doi: 10.1007/s13238-012-2002-0

A novel xeno-free and feeder-cell-free system for human pluripotent stem cell culture

doi: 10.1007/s13238-012-2002-0
Funds:

Grant Nos. 2006CB943901, 2010CB945024, and 2011CB965002), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-R-50), and the National Foundation of Science and Technology (No. 30640005).

Grant No. 2006AA02A106), the National Basic Research Program (973 Program

We thank members of the Pathology Lab at the Institute of Biophysics, CAS, for performing H.E. staining. This work was supported by the National High Technology Research and Development Program (863 Program

  • Received Date: 2011-11-02
  • Rev Recd Date: 2011-11-29
  • While human induced pluripotent stem cells (hiPSCs) have promising applications in regenerative medicine, most of the hiPSC lines available today are not suitable for clinical applications due to contamination with nonhuman materials, such as sialic acid, and potential pathogens from animal-product-containing cell culture systems. Although several xeno-free cell culture systems have been established recently, their use of human fibroblasts as feeders reduces the clinical potential of hiPSCs due to batch-to-batch variation in the feeders and time-consuming preparation processes. In this study, we have developed a xeno-free and feeder-cell-free human embryonic stem cell (hESC)/hiPSC culture system using human plasma and human placenta extracts. The system maintains the self-renewing capacity and pluripotency of hESCs for more than 40 passages. Human iPSCs were also derived from human dermal fibroblasts using this culture system by overexpressing three transcription factors-Oct4, Sox2 and Nanog. The culture system developed here is inexpensive and suitable for large scale production.
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