Volume 5 Issue 4
Apr.  2014
Turn off MathJax
Article Contents
Juan Ma, Huamin Han, Li Ma, Changzhen Liu, Xin Xue, Pan Ma, Xiaomei Li, Hua Tao. The immunostimulatory effects of retinoblastoma cell supernatant on dendritic cells[J]. Protein&Cell, 2014, 5(4): 307-316. doi: 10.1007/s13238-014-0029-0
Citation: Juan Ma, Huamin Han, Li Ma, Changzhen Liu, Xin Xue, Pan Ma, Xiaomei Li, Hua Tao. The immunostimulatory effects of retinoblastoma cell supernatant on dendritic cells[J]. Protein&Cell, 2014, 5(4): 307-316. doi: 10.1007/s13238-014-0029-0

The immunostimulatory effects of retinoblastoma cell supernatant on dendritic cells

doi: 10.1007/s13238-014-0029-0
Funds:

This work is funded by the grants from the Ministry of Science and Technology of China (S & T major Program:No. 2012ZX1004701-001-002), the National Basic Research Program (973 Program) (No. 2013CB531502), the National Nature Science Foundation of China (Grant Nos. 31170829, 31070783, and 81021003), and Beijing Natural Science Foundation (5112022).

  • Received Date: 2013-12-22
  • Rev Recd Date: 2014-01-20
  • Dendritic cells (DCs) are crucial for the induction and maintenance of tumor-specific immune responses. Studies have shown that tumor-associated DCs are immunosuppressed in some human tumors. However, phenotype and function of DCs in retinoblastoma (RB) remain unclear. RB cell supernatant (RBcs) was used to treat DCs in vitro to explore the effect of RB cells on DCs. DCs were generated from peripheral blood mononuclear cells of healthy donors. On day 5 of culture, DCs were treated with RBcs for 24 h, and then purified using magnetic beads. The maturation of DCs was induced by TNF-α or LPS. After treatment with RBcs, expression of co-stimulatory molecules CD80 and CD86 was elevated in DCs, accompanied by increased production of IL-12p70, TNF-α, IL-6, IL-1β, and IL-8 but decreased production of IL-10. RBcs neither inhibited DC maturation nor promoted DC apoptosis. Moreover, RBcs-exposed DCs stimulated allogenetic T cell proliferation and T cellderived cytokine production. These results indicate that RBcs can improve DCs' antigen presenting function and capability to activate T cells, suggesting that RB cells may have an immunostimulatory effect on DCs, and DCbased immunotherapy may be adopted in the treatment of RB.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (118) PDF downloads(5) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return