Volume 11 Issue 5
May  2020
Turn off MathJax
Article Contents
Peixuan Guo. High resolution structure of hexameric herpesvirus DNA-packaging motor elucidates revolving mechanism and ends 20-year fervent debate[J]. Protein&Cell, 2020, 11(5): 311-315. doi: 10.1007/s13238-020-00714-w
Citation: Peixuan Guo. High resolution structure of hexameric herpesvirus DNA-packaging motor elucidates revolving mechanism and ends 20-year fervent debate[J]. Protein&Cell, 2020, 11(5): 311-315. doi: 10.1007/s13238-020-00714-w

High resolution structure of hexameric herpesvirus DNA-packaging motor elucidates revolving mechanism and ends 20-year fervent debate

doi: 10.1007/s13238-020-00714-w

The research in Guo lab was supported by NIH grants R01EB019036, U01CA151648 and U01CA207946. Guo is the consultant of Oxford Nanopore Technologies

the cofounder of Shenzhen P&Z Bio-medical Co. Ltd, as well as cofounder of ExonanoRNA, LLC and its subsidiary Weina Biomedical LLC in Foshan. His Sylvan G. Frank Endowed Chair position in Pharmaceutics and Drug Delivery is funded by the CM Chen Foundation. Thanks to Zhefeng Li and Nicolas Burns for the preparation of the manuscript.

  • loading
  • [1]
    Acosta E, Bowlin T, Brooks J, Chiang L, Hussein I, Kimberlin D, Kauvar LM, Leavitt R, Prichard M, Whitley R (2020) Advances in the development of therapeutics for cytomegalovirus infections. J Infect Dis 221:S32-S44
    Arai S, Saijo S, Suzuki K, Mizutani K, Kakinuma Y, Ishizuka-Katsura Y, Ohsawa N, Terada T, Shirouzu M, Yokoyama S et al (2013) Rotation mechanism of Enterococcus hirae V1-ATPase based on asymmetric crystal structures. Nature 493:703-707
    Baumann RG, Mullaney J, Black LW (2006) Portal fusion protein constraints on function in DNA packaging of bacteriophage T4. Mol Microbiol 61:16-32
    Chang CL, Zhang H, Shu D, Guo P, Savran CA (2008) Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system. Appl Phys Lett 93:153902
    Chen W, Xiao H, Wang X, Song S, Han Z, Li X, Yang F, Wang L, Song, Liu H et al (2020) Structural changes of a bacteriophage upon DNA packaging and maturation. Protein Cell. https://doi.org/10.1007/s13238-020-00715-9
    Dong Y, Zhang S, Wu Z, Li X, Wang WL, Zhu Y, Stoilova-McPhie S, Lu Y, Finley D, Mao Y (2019) Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome. Nature 565:49-55
    Goldner T, Hewlett G, Ettischer N, Ruebsamen-Schaeff H, Zimmermann H, Lischka P (2011) The novel anticytomegalovirus compound AIC246 (Letermovir) inhibits human cytomegalovirus replication through a specific antiviral mechanism that involves the viral terminase. J Virol 85:10884-10893
    Guo P, Peterson C, Anderson D (1987a) Prohead and DNA-gp3-dependent ATPase activity of the DNA packaging protein gp16 of bacteriophage phi 29. J Mol Biol 197:229-236
    Guo PX, Erickson S, Anderson D (1987b) A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA. Science 236:690-694
    Guo P, Zhang C, Chen C, Garver K, Trottier M (1998) Inter-RNA interaction of phage phi29 pRNA to form a hexameric complex for viral DNA transportation. Mol Cell 2:149-155
    Guo P, Grainge I, Zhao Z, Vieweger M (2014) Two classes of nucleic acid translocation motors:rotation and revolution without rotation. Cell Biosci 4:54
    Guo P, Driver D, Zhao Z, Zheng Z, Chan C, Cheng X (2019) Controlling the revolving and rotating motion direction of asymmetric hexameric nanomotor by arginine finger and channel chirality. ACS Nano 13:6207-6223
    Hendrix RW (1978) Symmetry mismatch and DNA packaging in large bacteriophages. Proc Natl Acad Sci USA 75:4779-4783
    Hugel T, Michaelis J, Hetherington CL, Jardine PJ, Grimes S, Walter JM, Falk W, Anderson DL, Bustamante C (2007) Experimental test of connector rotation during DNA packaging into bacteriophage phi29 capsids. PLoS Biol 5:e59
    Jimenez J, Santisteban A, Carazo JM, Carrascosa JL (1986) Computer graphic display method for visualizing three-dimensional biological structures. Science 232:1113-1115
    Lyubimov AY, Costa A, Bleichert F, Botchan MR, Berger JM (2012) ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. Proc Natl Acad Sci USA 109:11999-12004
    Martin A, Baker TA, Sauer RT (2005) Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines. Nature 437:1115-1120
    Pi F, Vieweger M, Zhao Z, Wang S, Guo P (2016a) Discovery of a new method for potent drug development using power function of stoichiometry of homomeric biocomplexes or biological nanomotors. Expert Opin Drug Deliv 13:23-36
    Pi F, Zhao Z, Chelikani V, Yoder K, Kvaratskhelia M, Guo P (2016b) Development of potent antiviral drugs inspired by viral hexameric DNA-packaging motors with revolving mechanism. J Virol 90:8036-8046
    Puchades C, Rampello AJ, Shin M, Giuliano CJ, Wiseman RL, Glynn SE, Lander GC (2017) Structure of the mitochondrial inner membrane AAA+ protease YME1 gives insight into substrate processing. Science 358:eaao0464
    Schwartz C, De Donatis GM, Zhang H, Fang H, Guo P (2013) Revolution rather than rotation of AAA+ hexameric phi29 nanomotor for viral dsDNA packaging without coiling. Virology 443:28-39
    Shu D, Pi F, Wang C, Zhang P, Guo P (2015) New approach to develop ultra-high inhibitory drug using the power function of the stoichiometry of the targeted nanomachine or biocomplex. Nanomedicine 10:1881-1897
    Soultanas P, Wigley DB (2001) Unwinding the ‘Gordian knot’ of helicase action. Trends Biochem Sci 26:47-54
    Su M, Guo EZ, Ding X, Li Y, Tarrasch JT, Brooks CL 3rd, Xu Z, Skiniotis G (2017) Mechanism of Vps4 hexamer function revealed by cryo-EM. Sci Adv 3:e1700325
    Sun S, Li L, Yang F, Wang X, Fan F, Yang M, Chen C, Li X, Wang HW, Sui SF (2017) Cryo-EM structures of the ATP-bound Vps 4(E233Q) hexamer and its complex with Vta1 at near-atomic resolution. Nat Commun 8:16064
    Wang N, Zhao D, Wang J, Zhang Y, Wang M, Gao Y, Li F, Wang J, Bu Z, Rao Z et al (2019) Architecture of African swine fever virus and implications for viral assembly. Science 366:640-644
    Wang N, Chen W, Zhu L, Feng R, Wang J, Zhu D, Zhang X, Liu H, Rao Z, Wang X (2020) Structures of the portal vertex reveal essential protein-protein interactions for Herpesvirus assembly and maturation. Protein Cell. https://doi.org/10.1007/s13238-020-00711-z
    Yang Y, Yang P, Wang N, Zhu L, Zeng Y, Zhou ZH, Rao Z, Wang X (2020) Architecture of the herpesvirus genome-packaging complex and implications for DNA translocation. Protein Cell. https://doi.org/10.1007/s13238-020-00710-0
    Yuan S, Wang J, Zhu D, Wang N, Gao Q, Chen W, Tang H, Wang J, Zhang X, Liu H et al (2018) Cryo-EM structure of a herpesvirus capsid at 31 A. Science 360:eaao7283
    Zehr E, Szyk A, Piszczek G, Szczesna E, Zuo X, Roll-Mecak A (2017) Katanin spiral and ring structures shed light on power stroke for microtubule severing. Nat Struct Mol Biol 24:717-725
    Zhao Z, Khisamutdinov E, Schwartz C, Guo P (2013) Mechanism of one-way traffic of hexameric phi29 DNA packaging motor with four electropositive relaying layers facilitating antiparallel revolution. ACS Nano 7:4082-4092
    Zhao Z, De-Donatis GM, Schwartz C, Fang H, Li J, Guo P (2016) An arginine finger regulates the sequential action of asymmetrical hexameric ATPase in the double-stranded DNA translocation motor. Mol Cell Biol 36:2514-2523
    Zhu L, Sun Y, Fan J, Zhu B, Cao L, Gao Q, Zhang Y, Liu H, Rao Z, Wang X (2018) Structures of Coxsackievirus A10 unveil the molecular mechanisms of receptor binding and viral uncoating. Nat Commun 9:4985
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索


    Article Metrics

    Article views (700) PDF downloads(24) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint