Latest Articles

Articles in press have been peer-reviewed and accepted, which are not yet assigned to volumes/issues, but are citable by Digital Object Identifier (DOI).
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Single-cell RNA sequencing reveals B cell-T cell interactions in vascular adventitia of hyperhomocysteinemia-accelerated atherosclerosis
Xiaolong Ma, Jiacheng Deng, Lulu Han, Yuwei Song, Yutong Miao, Xing Du, Guohui Dang, Dongmin Yang, Bitao Zhong, Changtao Jiang, Wei Kong, Qingbo Xu, Juan Feng, Xian Wang
, Available online  , doi: 10.1007/s13238-021-00904-0
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A framework for the responsible reform of the 14-day rule in human embryo research
Yaojin Peng, Jianwei Lv, Zhenyu Xiao, Lulu Ding, Qi Zhou
, Available online  , doi: 10.1007/s13238-022-00907-5
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BMP4 preserves the developmental potential of mESCs through Ube2s- and Chmp4- bmediated chromosomal stability safeguarding
Mingzhu Wang, Kun Zhao, Meng Liu, Mengting Wang, Zhibin Qiao, Shanru Yi, Yonghua Jiang, Xiaochen Kou, Yanhong Zhao, Jiqing Yin, Tianming Li, Hong Wang, Cizhong Jiang, Shaorong Gao, Jiayu Chen
, Available online  , doi: 10.1007/s13238-021-00896-x
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Chemically defined medium is widely used for culturing mouse embryonic stem cells (mESCs), in which N2B27 works as a substitution for serum, and GSK3β and MEK inhibitors (2i) help to promote ground-state pluripotency. However, recent studies suggested that MEKi might cause irreversible defects that compromise the developmental potential of mESCs. Here, we demonstrated the deficient bone morphogenetic protein (BMP) signal in the chemically defined condition is one of the main causes for the impaired pluripotency. Mechanistically, activating the BMP signal pathway by BMP4 could safeguard the chromosomal integrity and proliferation capacity of mESCs through regulating downstream targets Ube2s and Chmp4b. More importantly, BMP4 promotes a distinct in vivo developmental potential and a long-term pluripotency preservation. Besides, the pluripotent improvements driven by BMP4 are superior to those by attenuating MEK suppression. Taken together, our study shows appropriate activation of BMP signal is essential for regulating functional pluripotency and reveals that BMP4 should be applied in the serumfree culture system.
mTORC2/RICTOR exerts differential levels of metabolic control in human embryonic, mesenchymal and neural stem cells
Qun Chu, Feifei Liu, Yifang He, Xiaoyu Jiang, Yusheng Cai, Zeming Wu, Kaowen Yan, Lingling Geng, Yichen Zhang, Huyi Feng, Kaixin Zhou, Si Wang, Weiqi Zhang, Guang-Hui Liu, Shuai Ma, Jing Qu, Moshi Song
, Available online  , doi: 10.1007/s13238-021-00898-9
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Correction to: Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction
Si Wang, Zheying Min, Qianzhao Ji, Lingling Geng, Yao Su, Zunpeng Liu, Huifang Hu, Lixia Wang, Weiqi Zhang, Keiichiro Suzuiki, Yu Huang, Puyao Zhang, Tie-Shan Tang, Jing Qu, Yang Yu, Guang-Hui Liu, Jie Qiao
, Available online  , doi: 10.1007/s13238-021-00901-3
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Unveiling E2F4, TEAD1 and AP-1 as regulatory transcription factors of the replicative senescence program by multi-omics analysis
Yuting Wang, Liping Liu, Yifan Song, Xiaojie Yu, Hongkui Deng
, Available online  , doi: 10.1007/s13238-021-00894-z
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Senescence, a stable state of growth arrest, affects many physiological and pathophysiological processes, especially aging. Previous work has indicated that transcription factors (TFs) play a role in regulating senescence. However, a systematic study of regulatory TFs during replicative senescence (RS) using multiomics analysis is still lacking. Here, we generated timeresolved RNA-seq, reduced representation bisulfite sequencing (RRBS) and ATAC-seq datasets during RS of mouse skin fibroblasts, which demonstrated that an enhanced inflammatory response and reduced proliferative capacity were the main characteristics of RS in both the transcriptome and epigenome. Through integrative analysis and genetic manipulations, we found that transcription factors E2F4, TEAD1 and AP-1 are key regulators of RS. Overexpression of E2f4 improved cellular proliferative capacity, attenuated SA-β-Gal activity and changed RS-associated differentially methylated sites (DMSs). Moreover, knockdown of Tead1 attenuated SA-β-Gal activity and partially altered the RS-associated transcriptome. In addition, knockdown of Atf3, one member of AP-1 superfamily TFs, reduced Cdkn2a (p16) expression in pre-senescent fibroblasts. Taken together, the results of this study identified transcription factors regulating the senescence program through multi-omics analysis, providing potential therapeutic targets for anti-aging.
8 Å structure of the outer rings of the Xenopus laevis nuclear pore complex obtained by cryo-EM and AI
Linhua Tai, Yun Zhu, He Ren, Xiaojun Huang, Chuanmao Zhang, Fei Sun
, Available online  , doi: 10.1007/s13238-021-00895-y
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The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 Å resolution cryo-electron microscopic (cryo-EM) structure of the outer rings containing nuclear ring (NR) and cytoplasmic ring (CR) from the Xenopus laevis NPC, with local resolutions reaching 4.9 Å. With the aid of AlphaFold2, we managed to build a pseudoatomic model of the outer rings, including the Y complexes and flanking components. In this most comprehensive and accurate model of outer rings to date, the almost complete Y complex structure exhibits much tighter interaction in the hub region. In addition to two copies of Y complexes, each asymmetric subunit in CR contains five copies of Nup358, two copies of the Nup214 complex, two copies of Nup205 and one copy of newly identified Nup93, while that in NR contains one copy of Nup205, one copy of ELYS and one copy of Nup93. These in-depth structural features represent a great advance in understanding the assembly of NPCs.
Modeling hepatoblastoma development with human fetal liver organoids reveals YAP1 activation is sufficient for tumorigenesis
Li Yang, Jin Chen, Jianqing Liang, Yufeng Zhang, Qingzhe Wang, Xiaojun Ren, Jinsong Wei, Qianchun Gong, Jiting Zhang, Ning Jiang, Xinhua Lin, Jin Li, Bing Zhao
, Available online  , doi: 10.1007/s13238-021-00893-0
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Academician Qi-Yi Xing (Chi-Yi Hsing): pioneer organic chemist of synthetic insulin
Ming-Hua Qiu
, Available online  , doi: 10.1007/s13238-021-00891-2
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Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation
Guang Shi, Yaofu Bai, Xiya Zhang, Junfeng Su, Junjie Pang, Quanyuan He, Pengguihang Zeng, Junjun Ding, Yuanyan Xiong, Jingran Zhang, Jingwen Wang, Dan Liu, Wenbin Ma, Junjiu Huang, Zhou Songyang
, Available online  , doi: 10.1007/s13238-021-00884-1
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Hyperthermia differentially affects specific human stem cells and their differentiated derivatives
Si Wang, Fang Cheng, Qianzhao Ji, Moshi Song, Zeming Wu, Yiyuan Zhang, Zhejun Ji, Huyi Feng, Juan Carlos Izpisua Belmonte, Qi Zhou, Jing Qu, Wei Li, Guang-Hui Liu, Weiqi Zhang
, Available online  , doi: 10.1007/s13238-021-00887-y
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Crystal structure of SARS-CoV-2 main protease in complex with protease inhibitor PF-07321332
Yao Zhao, Chao Fang, Qi Zhang, Ruxue Zhang, Xiangbo Zhao, Yinkai Duan, Haofeng Wang, Yan Zhu, Lu Feng, Jinyi Zhao, Maolin Shao, Xiuna Yang, Leike Zhang, Chao Peng, Kailin Yang, Dawei Ma, Zihe Rao, Haitao Yang
, Available online  , doi: 10.1007/s13238-021-00883-2
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An ultrapotent pan-β-coronavirus lineage B (β-CoV-B) neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope
Zezhong Liu, Wei Xu, Zhenguo Chen, Wangjun Fu, Wuqiang Zhan, Yidan Gao, Jie Zhou, Yunjiao Zhou, Jianbo Wu, Qian Wang, Xiang Zhang, Aihua Hao, Wei Wu, Qianqian Zhang, Yaming Li, Kaiyue Fan, Ruihong Chen, Qiaochu Jiang, Christian T. Mayer, Till Schoofs, Youhua Xie, Shibo Jiang, Yumei Wen, Zhenghong Yuan, Kang Wang, Lu Lu, Lei Sun, Qiao Wang
, Available online  , doi: 10.1007/s13238-021-00871-6
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New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design. Here, we identified a receptor-binding domain (RBD)-binding antibody, XG014, which potently neutralizes β-coronavirus lineage B (β-CoV-B), including SARS-CoV-2, its circulating variants, SARS-CoV and bat SARSr-CoV WIV1. Interestingly, antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibody-dependent SARS-CoV-2 spike (S) protein-mediated cell-cell fusion, suggesting a unique mode of recognition by XG014. Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional “down” conformation, while its family member XG005 directly competes with ACE2 binding and position the RBD “up”. Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo. Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines against β-CoV-B and newly emerging SARS-CoV-2 variants of concern.
Large-scale chemical screen identifies Gallic acid as a geroprotector for human stem cells
Hezhen Shan, Lingling Geng, Xiaoyu Jiang, Moshi Song, Jianxun Wang, Zunpeng Liu, Xiao Zhuo, Zeming Wu, Jianli Hu, Zhejun Ji, Si Wang, Piu Chan, Jing Qu, Weiqi Zhang, Guang-Hui Liu
, Available online  , doi: 10.1007/s13238-021-00872-5
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Legacy of Hongxi Su: Pioneer of Chinese Cardiosurgery
Sumin Yang, Haoyu Hu, Xin Zheng, Shizhong Wang, Li Yuan
, Available online  , doi: 10.1007/s13238-021-00848-5
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The mini player with diverse functions: extracellular vesicles in cell biology, disease, and therapeutics
Abhimanyu Thakur, Xiaoshan Ke, Ya-Wen Chen, Pedram Motallebnejad, Kui Zhang, Qizhou Lian, Huanhuan Joyce Chen
, Available online  , doi: 10.1007/s13238-021-00863-6
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LIN28 coordinately promotes nucleolar/ ribosomal functions and represses the 2C-like transcriptional program in pluripotent stem cells
Zhen Sun, Hua Yu, Jing Zhao, Tianyu Tan, Hongru Pan, Yuqing Zhu, Lang Chen, Cheng Zhang, Li Zhang, Anhua Lei, Yuyan Xu, Xianju Bi, Xin Huang, Bo Gao, Longfei Wang, Cristina Correia, Ming Chen, Qiming Sun, Yu Feng, Li Shen, Hao Wu, Jianlong Wang, Xiaohua Shen, George Q. Daley, Hu Li, Jin Zhang
, Available online  , doi: 10.1007/s13238-021-00864-5
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Emerging roles of spliceosome in cancer and immunity
Hui Yang, Bruce Beutler, Duanwu Zhang
, Available online  , doi: 10.1007/s13238-021-00856-5
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Metabolic reprogramming and epigenetic modifications on the path to cancer
Linchong Sun, Huafeng Zhang, Ping Gao
, Available online  , doi: 10.1007/s13238-021-00846-7
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Darwinian rebel: The legacy of Loh-Seng Tsai—Chinese animal psychologist
Yong Wang, Wei Chen, Shiying Li, Bin Yin
, Available online  , doi: 10.1007/s13238-021-00849-4
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Genomic and transcriptomic analysis unveils population evolution and development of pesticide resistance in fall armyworm Spodoptera frugiperda
Furong Gui, Tianming Lan, Yue Zhao, Wei Guo, Yang Dong, Dongming Fang, Huan Liu, Haimeng Li, Hongli Wang, Ruoshi Hao, Xiaofang Cheng, Yahong Li, Pengcheng Yang, Sunil Kumar Sahu, Yaping Chen, Le Cheng, Shuqi He, Ping Liu, Guangyi Fan, Haorong Lu, Guohai Hu, Wei Dong, Bin Chen, Yuan Jiang, Yongwei Zhang, Hanhong Xu, Fei Lin, Bernard Slipper, Alisa Postma, Matthew Jackson, Birhan Addisie Abate, Kassahun Tesfaye, Aschalew Lemma Demie, Meseret Destaw Bayeleygne, Dawit Tesfaye Degefu, Feng Chen, Paul K. Kuria, Zachary M. Kinyua, Tong-Xian Liu, Huanming Yang, Fangneng Huang, Xin Liu, Jun Sheng, Le Kang
, Available online  , doi: 10.1007/s13238-020-00795-7
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A world renowned psychophysiologist: Kaoliang Chow
Lei Zhang, Lijun Wang, Benyu Guo, Yanyan Qian, Qingming Liu
, Available online  , doi: 10.1007/s13238-020-00797-5
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Chia Chi Wang and his research on Protozoology
Ting Shi, Lei Fu
, Available online  , doi: 10.1007/s13238-020-00759-x
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Tsai Chiao: The founder of physiology and aviation, aerospace and navigation medicine in China
Yalan Wang, Zijian Li, Yanyan Qian, Benyu Guo
, Available online  , doi: 10.1007/s13238-020-00737-3
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Qi Fang: Affective interweave with patients
Tanping Fu, Wenli Duan, Mingyan Chen, Shuyang Zhang
, Available online  , doi: 10.1007/s13238-019-00667-9
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Correction to: Developing potent PROTACs tools for selective degradation of HDAC6 protein
Zixuan An, Wenxing Lv, Shang Su, Wei Wu, Yu Rao
, Available online  , doi: 10.1007/s13238-019-0613-4
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Professor Li-Pin. King: a famous physiologist in China
Wei Gong, Fangfang Wang, Yike Ying
, Available online  , doi: 10.1007/s13238-018-0582-z
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Retraction Note to: DDB1 and Cul4 are necessary for gene silencing and heterochromatin formation at pericentromeric regions in Neurospora
Yingqiong Cao, Jicheng Wei, Silu Yang, Jinquan Sun, Hui Xu, Ying Wang, Yuanbiao Zhao, Qun He
, Available online  , doi: 10.1007/s13238-014-0079-3
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RETRACTED ARTICLE: DDB1 and Cul4 are necessary for gene silencing and heterochromatin formation at pericentromeric regions in Neurospora
Yingqiong Cao, Jicheng Wei, Silu Yang, Jinquan Sun, Hui Xu, Ying Wang, Yuanbiao Zhao, Qun He
, Available online  , doi: 10.1007/s13238-014-0067-7
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